Metabolic engineering
PET-FBA: A lightweight enzyme allocation and thermodynamics-constrained flux analysis approach to explore Escherichia coli metabolic adaptation to intracellular acidification.
Chao Wu, Jeffrey N Law, Onyeka Onyenemezu, Jetendra K Roy, Peter C St John, Robert L Jernigan, Yannick J Bomble, Laura Jarboe
Published: 202510.1016/j.ymben.2025.12.003
Abstract
Escherichia coli employs diverse strategies to adapt to acidic environments that disrupt enzyme activity and the thermodynamic feasibility of essential reactions. To understand the impact of pH stress on cell metabolism, we present the PET-FBA (pH-,…
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