Metabolic engineeringHumansAmmoniaHEK293 CellsCRISPR-Cas SystemsAntibodies
Genome-wide CRISPR screening identifies genes in recombinant human embryonic kidney 293 cells for increased ammonia resistance.
Sang Yoon Lee, Hyun Seung Kim, Yeon Gu Kim, Seunghyeon Shin, Seokchan Kweon, Jae Jun Lee, Gyun Min Lee
Published: 202610.1016/j.ymben.2025.10.008
Abstract
Ammonia, a byproduct of glutamine metabolism, inhibits cell growth and reduces product yield and quality in mammalian cell culture. To identify novel genes associated with ammonia resistance, a genome-wide CRISPR knockout screening was conducted in m…
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