STAR protocols
Protocol to investigate human mitochondrial transcription initiation by integrating biochemical and cryo-EM approaches.
Yogeeshwar Ajjugal, Quinten Goovaerts, Jiayu Shen, Brent De Wijngaert, Kalyan Das, Smita S Patel
Published: 202610.1016/j.xpro.2025.104347
Abstract
Here, we present a protocol to investigate the mechanism and regulation of human mitochondrial transcription initiation by integrating biochemical and cryoelectron microscopy (cryo-EM) approaches. We describe the steps for expressing and purifying PO…
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