Protocol for retinal stress culture and single-cell analysis.

The retina is a dynamic neural tissue that lines the posterior of the eye cup and transfers visual inputs from the world to our brain for processing. However, exposure to stress, injury, and disease can disrupt this important function. Here, we present a protocol for the dissection and culture of retina in various physiologically relevant stress conditions. Furthermore, we describe how to assess the retina for stress and cell-type-specific responses. For complete details on the use and execution of this protocol, please refer to Norrie et al.1.