TheriogenologyAnimalsCattleFemaleGene EditingElectroporation
Efficient genome editing of bovine in vitro derived zygotes via Cas9 RNP-electroporation using extended-stored bovine ovaries.
M Rahimi, M Schreiber, H Habermann, L Haake, D Miskel, D Tesfaye, E Held-Hoelker, B Brenig, C Benedetti, M Hoelker
Published: 202610.1016/j.theriogenology.2025.117703
Abstract
Bovine embryos serve as a relevant model for human preimplantation development and are increasingly used in genome editing research. Although CRISPR-Cas9 electroporation in zygotes offers a promising approach, challenges with respect to editing effic…
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