Stem cell researchHumansCRISPR-Cas SystemsHuman Embryonic Stem CellsGene Knockout TechniquesRetinoblastoma Binding Proteins
Generation of RB1 knockout human embryonic stem cell lines derived from H9 using CRISPR/Cas9.
Jinyi Wang, Yimeng Gao, Kangxin Jin, Zi-Bing Jin, Jia Xu
Published: 202610.1016/j.scr.2025.103897
Abstract
RB1 is a tumor suppressor gene which plays a crucial role in cell cycle and cellular differentiation. Mutations or loss of RB1 are associated with retinoblastoma and a variety of other cancers. We generated RB1 knockout human embryonic stem cell (hES…
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