Protein expression and purificationGranulocyte Colony-Stimulating FactorEscherichia coliFermentationRecombinant ProteinsProtein Refolding
Effect of culture media and fermentation process on the refolding and purification of rh-GCSF.
Somayeh Abolghasemi, Fatemeh Poureini, Valiollah Babaeipour, Faezeh Farji, Mohammad Reza Mofid
Published: 202610.1016/j.pep.2025.106837
Abstract
Human granulocyte-colony stimulating factor (h-GCSF) is used to mitigate neutropenia caused by chemotherapy. E. coli is the most common host for h-GCSF production due to its high yield and versatile culture strategies. However, overproduction of h-GC…
Preview only. Read the full abstract at the source