Journal of neuroscience methodsChannelrhodopsinsOptogeneticsSingle-Cell AnalysisAnimalsHumans
Screening channelrhodopsins using robotic intracellular electrophysiology and single cell sequencing.
Samuel Ehrlich, Alexandra D VandeLoo, Mohamed Badawy, Mercedes M Gonzalez, Max Stockslager, Aimei Yang, Sapna Sinha, Shahar Bracha, Demian Park, Benjamin Magondu, Bo Yang, Edward S Boyden, Craig R Forest
Published: 202610.1016/j.jneumeth.2025.110663
Abstract
BACKGROUND: Our ability to engineer opsins is limited by an incomplete understanding of how sequence variations influence function. The vastness of opsin sequence space makes systematic exploration difficult. NEW METHOD: In recognition of the need fo…
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