Journal of biotechnologyInclusion BodiesEscherichia coliPressureProtein RefoldingRecombinant Proteins
Increased purity and refolding yield of bacterial inclusion bodies by recursive high pressure homogenization.
Robert Klausser, Lukas Veiter, Julian Kopp, Nikolaus Hammerschmidt, Tiffany Frierss, Florian Gisperg, Mohamed Elshazly, Eva Prada Brichtova, Michael Martinetz, Martin Voigtmann, Oliver Spadiut
Published: 202610.1016/j.jbiotec.2025.11.004
Abstract
Therapeutic proteins are often produced intracellularly in the bacterium Escherichia coli in aggregated form, known as inclusion bodies, which must be solubilized and refolded to recover the biologically active product. However, the industrially esta…
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