Journal of bioscience and bioengineering
Single-cell ATP monitoring in Escherichia coli engineered for polyhydroxybutyrate production using the fluorescent biosensor QUEEN.
Wataru Fuji, Ayaka Kajikawa, Shin-Ichi Hachisuka, Hiroya Tomita, Hiroshi Kikukawa, Hiromi Imamura, Ken'ichiro Matsumoto
Published: 202610.1016/j.jbiosc.2025.12.004
Abstract
Polyhydroxyalkanoates (PHAs) are polyesters accumulated by various microorganisms as intracellular inclusions. In this study, Escherichia coli was engineered to co-express PHA biosynthetic genes and the ratiometric fluorescent biosensor QUEEN. QUEEN…
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