International journal of biological macromoleculesD-Amino-Acid OxidaseProtein EngineeringEnzyme StabilityMolecular Dynamics SimulationSubstrate Specificity
Development of a highly functional thermostable D-amino acid oxidase for efficient L-phosphinothricin biosynthesis using ProteinMPNN-guided engineering.
Kai Yang, Yueshan Huang, Liyi Yao, Bin Huang, Weimin Zeng, Jin Huang
Published: 202610.1016/j.ijbiomac.2025.149737
Abstract
D-amino acid oxidase (DAAO) has long been recognized as a promising catalyst for chiral amino acid biosynthesis owing to its pronounced stereospecificity. However, its limited thermostability restricts industrial utility and poses challenges for prot…
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