International journal of biological macromoleculesEscherichia coliFermentationBacterial ProteinsSolubilityRecombinant Proteins
Efficient bioprocess for high-density fermentation and rapid purification of soluble tag-free CRM197 protein expressed in Escherichia coli.
Shubham Singh, Jaideep Mehta, Ravi P N Mishra
Published: 202610.1016/j.ijbiomac.2025.149685
Abstract
Cross-reacting material 197, or CRM197, is a genetically detoxified variant of diphtheria toxin that is an integral part of several approved polysaccharide-conjugate vaccines against encapsulated pathogenic bacteria. CRM197 is a high-value protein ne…
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