International journal of biological macromoleculesSaccharomyces cerevisiaeGene EditingRNAGuideCRISPR-Cas Systems
Engineered sgRNA captures single-stranded donor template and delivers at the DSB site to enhance HDR.
Somnath Rahangdale, Ashish Vishwakarma, Ranjana Chauhan, Sahil Singh, Pradhyumna Kumar Singh
Published: 202510.1016/j.ijbiomac.2025.148614
Abstract
The CRISPR-Cas9 system from Streptococcus pyogenes has revolutionized genome modification through precise editing across a wide range of organisms. Yeast supports efficient genome editing via plasmid-based Cas9-gRNA expression, while higher eukaryote…
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