International journal of biological macromoleculesCationsChemical PrecipitationProtein EngineeringAmino Acids
Engineered oligomeric tags confer cation responsiveness and reversible precipitation ability to DsRed, facilitating its purification.
Hongxu Sun, Chao Gao, Ruping Lu, Yushang Zhou, Hongrui Wang, Yanhong Chang, Hui Luo
Published: 202510.1016/j.ijbiomac.2025.148417
Abstract
Fusion tags and intrinsic surface residues on proteins interact intricately with ions in solution. These interactions can be harnessed to enable protein purification, while uncontrolled interactions may lead to undesired aggregation or precipitation.…
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