Impact of harvest periods of Sichuan pepper on Sichuan pepper-flavoured chicken using electronic nose, electronic tongue, and chromatography.
Lian He, Zongyuan Lu, Xuemei Cai, Kaixian Zhu, Chengjian Xu, Dan Lin, Zhiyong Hou, Sze Ying Leong, Nallammai Singaram, Cui Yang, Sook Wah Chan, Hua Peng, Minglu Lan
Abstract
Open AccessThis study compared Sichuan pepper harvested at five stages (July-September) for preparing Sichuan pepper-flavoured chicken. Electronic nose and tongue analyses separated the control from peppered samples and further distinguished early (July-august) from late (September) harvests; September pepper produced stronger odour, higher umami and sourness, and lower bitterness. GC-IMS fingerprints revealed harvest-dependent shifts in volatile profiles, and OPLS-DA (VIP > 1) highlighted limonene-D/M (highest VIP), terpinolene, myrcene, sec-butanol, isobutyl acetate-D, and terpinyl acetate-D/M as key markers. HS-GC-MS detected 68 volatiles; OPLS-DA markers (VIP > 1) included 3-penten-2-ol (highest VIP), β-pinene, limonene, phellandrene, linalyl acetate, and eucalyptol, while α-pinene varied significantly with harvest period. Overall, later harvest increased terpene/ester abundance and most effectively enhanced the characteristic flavour of Sichuan pepper-flavoured chicken.