Enzyme and microbial technology
Efficient production of active recombinant cholesterol oxidase from Rhodococcus erythropolis in Escherichia coli via his-tag assisted refolding strategy for cholesterol oxidation.
Meka Saima Perdani, Dwini Normayulisa Putri, Dita Ariyanti, Cintiya Septa Hasannah, Fitri Yuliasari, Ibnu Maulana Hidayatullah, Heri Hermansyah, Yosuke Fukutani, Masafumi Yohda
Published: 202510.1016/j.enzmictec.2025.110805
Abstract
This study reports the cloning, expression, and characterization of recombinant cholesterol oxidase (ChOx) from Rhodococcus erythropolis in Escherichia coli BL21(DE3). The ChOx gene was inserted into the pET23b(+) vector using primers designed with s…
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