Enzyme and microbial technologyFluorescent DyesFluorometryThiolester HydrolasesGlutathioneEnzyme Assays
Sensitive and specific fluorometric assay for assessment of glyoxalase II enzymatic activity in microbial samples and biological tissue.
Mohammed Alaa Kadhum, Mahmoud Hussein Hadwan
Published: 202610.1016/j.enzmictec.2025.110783
Abstract
This study presents a novel fluorescence-based assay for quantifying Glyoxalase II (Glo II) enzymatic activity, using N-(9-Acridinyl)maleimide (NAM) as a fluorescent probe. The assay is designed to measure glutathione (GSH) production resulting from…
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