Journal of chromatography. A
Decoding diastereomeric and impurity profiles of phosphorothioated siRNAs via 2D anion-exchange chromatography coupled with ion-pair reversed phase liquid chromatography mass spectrometry.
Quang-Dong Bui, Willy Verluyten, Bart Noten, Tiny Deschrijver, Sebastiaan Eeltink
Published: 202510.1016/j.chroma.2025.466639
Abstract
Phosphorothioate (PS) modifications are widely used in RNA-based therapeutic oligonucleotides (ONs), forming diastereomers that enhance ON stability, bioavailability, and cellular uptake. This study presents a strategy for profiling PS-ONs, including…
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