Cell systemsBenchmarkingHumansTandem Mass SpectrometryMass SpectrometryChromatography
Systematic benchmarking of mass spectrometry-based antibody sequencing reveals methodological biases.
Maria Chernigovskaya, Khang Lê Quý, Maria Stensland, Sachin Singh, Rowan Nelson, Melih Yilmaz, Konstantinos Kalogeropoulos, Pavel Sinitcyn, Anand Patel, Natalie Castellana, Stefano Bonissone, Stian Foss, Jan Terje Andersen, Geir Kjetil Sandve, Timothy Patrick Jenkins
Published: 202510.1016/j.cels.2025.101449
Abstract
The circulating antibody (Ab) repertoire is crucial for immune protection, holding significant immunological and biotechnological value. While bottom-up mass spectrometry (MS) is widely used for profiling the sequence diversity of circulating Abs (Ab…
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