Cell systemsStreptococcus pneumoniaeCell CycleCRISPR-Cas SystemsGenomeBacterial
Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle.
Julien Dénéréaz, Elise Eray, Bimal Jana, Vincent de Bakker, Horia Todor, Tim van Opijnen, Xue Liu, Jan-Willem Veening
Published: 202510.1016/j.cels.2025.101408
Abstract
Uncovering genotype-phenotype relationships is hampered by genetic redundancy. For example, most genes in Streptococcus pneumoniae are non-essential under laboratory conditions. A powerful approach to unravel genetic redundancy is by identifying gene…
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