Biosensors & bioelectronicsCRISPR-Cas SystemsNucleic Acid Amplification TechniquesSARS-CoV-2Biosensing TechniquesHumans
Ultra-fast one-pot isothermal detection of respiratory virus: ADNA-initiated CRISPR/Cas12a-mediated RCA cycle.
Junzhuo Shan, Wenhai Wang, Yixiao Sheng, Bin Hong, Lun Luo, Xinjiang Liu, Yi Ma, Jufang Wang
Published: 202610.1016/j.bios.2025.118141
Abstract
Respiratory virus detection is a prominent area of molecular diagnostics, yet current clinical diagnostic methods lack ultra-rapid and highly sensitive detection capabilities. Here, we reported an ultra-rapid, one-pot isothermal assay called "ADNA-in…
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