Bioorganic chemistryProtein EngineeringAlcohol OxidoreductasesAmino AcidsStereoisomerismMolecular Dynamics Simulation
Cavity engineering-driven switching of cofactor specificity in a carbonyl reductase for the synthesis of chiral aryl β-Hydroxy α-amino acid.
Jiawei Tang, Jirui Zhong, Hao Chen, Luwen Zhang, Junxi Shao, Haodong Chen, Fuli Zhang, Hongchun Li, Shaoxin Chen
Published: 202510.1016/j.bioorg.2025.109295
Abstract
Carbonyl reductases (CRs) are important enzymes with a broad spectrum of industrial applications. However, the CRs reported for use in the asymmetric synthesis of chiral aryl β-hydroxy α-amino acids are exclusively specific to NADPH, posing challenge…
Preview only. Read the full abstract at the source