Bioorganic chemistryCloningMolecularCold TemperaturePeptide HydrolasesHydrogen-Ion Concentration
Gene cloning, expression and characterization of a novel cold-adapted protease from Colwellia hornerae.
Xi Xie, Jiansheng Lin, Li Lin, Yuhan Guo, Xiaozhi Lin, Yingxi Cai, Gengsheng Xiao, Qin Wang, Keke Li, Huifan Liu
Published: 202510.1016/j.bioorg.2025.109141
Abstract
A cold-adapted protease (Chprotease) from Colwellia hornerae, identified through genomic screening, was cloned, heterologously expressed and purified. The purified Chprotease had a molecular weight of 53.3 kDa and showed maximum specific activity (35…
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