Analytical biochemistryTrypsinMass SpectrometryNitrogenaseMetalloproteinsChemical Precipitation
Trypsin digestion to prevent acid induced precipitation of metalloproteins for accurate ICP-MS analysis: Nitrogenase case study.
Jessica K Bilyj, Christina M Gregg, Trevor D Rapson
Published: 202610.1016/j.ab.2025.116035
Abstract
Accurate determination of metallation states in metalloenzymes is essential for correlating metal content with enzymatic function. Inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectroscopy (ICP-OES) are among the most se…
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