Analytical biochemistryInclusion BodiesNEDD8 ProteinProtein RefoldingEscherichia coliDialysis
A minimal slow dialysis method for refolding inclusion body proteins: Structural application to NEDD8 and its Q40E mutant.
Anna Makovska, Erik Walinda, Ryo Aoyama, Minsoo Kim, Daichi Morimoto
Published: 202610.1016/j.ab.2025.116009
Abstract
NEDD8, a ubiquitin-like modifier essential for cullin-RING ligase regulation, is difficult to refold from Escherichia coli inclusion bodies due to aggregation during denaturant removal. Conventional refolding methods such as rapid dilution, one-step…
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