Methods in enzymologyPseudomonas aeruginosaMembrane Transport ProteinsBacterial Outer Membrane ProteinsSingle Molecule ImagingMicroscopy
Reconstitution of RND tripartite multidrug complexes for single-particle electron microscopy.
Esther Boyer, Laetitia Daury, Marie-France Giraud, Marie-Christine Gerbod-Giannone, Sylvie Poussard, Isabelle Broutin, Jean-Christophe Taveau, Olivier Lambert
Published: 202510.1016/bs.mie.2025.08.024
Abstract
Tripartite multidrug RND efflux pumps (TEP) made of an inner membrane RND transporter, an outer membrane factor (OMF) and a periplasmic adaptor protein (PAP) form a canal for expelling drugs through the cell wall of Gram-negative bacteria. Various RN…
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