Molecular biotechnology
Efficient Construction of Recombinant CHO Cells for Stable Expression of Therapeutic Proteins Based on New Hotspot 'LOC'.
Yun Chen, Ziyu Wang, Xia Lei, Yazhou Liu, Yanfei Cai, Chengfei Wang, Jian Jin, Xuefeng Ding
Published: 202510.1007/s12033-025-01506-5
Abstract
Efficient generation of stably express therapeutic recombinant proteins in Chinese hamster ovary (CHO) cell lines remains as a major challenge in biologics development. Among available strategies, CRISPR/Cas9-mediated homology-directed repair (HDR) h…
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