Urinary metabolome dynamics in 13C-labeled mice.

INTRODUCTION: Analysis of specific pathways of metabolic flux is usually achieved by administering stable isotope-labeled precursors and measuring their incorporation into selected metabolites by high-resolution mass spectrometry coupled to liquid chromatography (LC-HRMS). OBJECTIVES: In this study, we undertook a novel approach aiming at covering the whole metabolome dynamics by providing mice with a diet fully enriched in carbon-13 (13C). METHODS: Three animals were fed for six weeks with small pellets composed of a mixture of 13C-spirulina and 13C-wheat. All animals grew normally and their urine was collected daily. Three control mice were treated in the same way, but with an unenriched diet. LC-HRMS-based metabolomics profiling were conducted on all collected samples as well as isotope tracing. RESULTS: Comparative LC-HRMS analysis of the 12C- and 13C-samples unambiguously identified 238 metabolites, whose 13C-labeling profiles were then studied over a 39-day period. Although overall urine labeling was fast and rapidly reached a high level of 13C-content (> 90% after 22 days), isotopic monitoring of each molecular species demonstrated that 13C-incorporation kinetics are considerably variable between metabolites, reflecting their biological function, origin and rate of biosynthesis in vivo. CONCLUSION: This study demonstrates that mice tolerate a diet entirely labeled with carbon-13 (> 97 atom % 13C) over a period of six-weeks. It reveals the dynamics of a large part of the metabolome at the mammalian species level through urine analysis coupled to in vivo 13C-labeling. The study provides valuable insights by comprehensively covering metabolic pathways, and stands out from targeted fluxomic studies which are carried out by administering a very limited number of specific labeled precursors.