Biotechnology lettersAlkenesAcinetobacterCloningMolecularEscherichia coli
Cloning and characterization of a new oleate hydratase from Acinetobacter ursingii for asymmetric hydration of unactivated alkenes.
Xuan Deng, Yu Wan, Yunyi Li, Xiangyu Wang, Hang Gao, Juan Zhang, Wenchao Gao, Shuangping Huang, Lili Gao, Jiandong Zhang
Published: 202510.1007/s10529-025-03670-9
Abstract
OBJECTIVES: To expand the availability of promiscuous oleate hydratases (OAHs) for the asymmetric hydration of unactivated alkenes via sequence-based genome mining combined with targeted amino acid substitution. RESULTS: From 100 screened OAHs, 13 ca…
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