β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for periodontal regeneration in class II furcation defects in dogs.

OBJECTIVES: The present study aimed to evaluate the effect of β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for periodontal regeneration in Class II Furcation Defects in Dogs. MATERIALS AND METHODS: 24 bilateral class II furcation defects, 5 mm height, and 3 mm depth, were surgically created on the buccal surface of mandibular premolars and allowed to become chronically healed in six mongrel male dogs. Dogs were randomly divided into three groups: Group I received β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres (CMs (GM)-β-TCP/gelatin composite scaffolds). Group II received the same scaffolds without gentamycin. Group III received open flap debridement (OFD) as a control. The animals were sacrificed at 8 weeks post-operatively for histological and histomorphometry analysis (n = 8, α = 0.05). RESULTS: New regenerative tissue was significantly (P < .001) greater in both the test groups, as they showed the highest percentage of newly formed bone height, newly formed bone area, newly formed cementum, and newly formed periodontal tissues than the control. At the same time, epithelial down growth was predominant in the control group. CONCLUSION: Within the limitations of this study, the novel scaffolds improved periodontal regeneration and wound healing in chronic class II furcation defects. CLINICAL RELEVANCE: The Bone regeneration property of (CMs (GM)-β-TCP/gelatin composite scaffolds) is implicated in periodontal bone defect treatment and pre-implant alveolar bone regeneration.