Plant cell reportsMusaCRISPR-Cas SystemsGene Knock-In TechniquesGene EditingGreen Fluorescent Proteins
CRISPR/Cas9-mediated gene targeting at BBM2 locus demonstrates HDR-assisted precise knock-in in banana cv. Grand Naine.
Surender Singh, Roni Chaudhary, Siddharth Tiwari
Published: 202510.1007/s00299-025-03654-z
Abstract
KEY MESSAGE: The present study demonstrates the first CRISPR/Cas-mediated precise knock-in of the eGFP gene at the BABYBOOM2 (GN-BBM2) locus in banana cv. Grand Naine, facilitating the detection of editing events in early embryogenic developmental st…
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