Methods in molecular biology (Clifton, N.J.)PhytoplasmaCRISPR-Cas SystemsPlant DiseasesNucleic Acid Amplification TechniquesRecombinases
Rapid and Sensitive Detection of Phytoplasma Diseases Using a CRISPR/Cas12a DETECTR Assay Combined with Isothermal Recombinase Polymerase Amplification.
Wei Wei, Yinong Yang, Justin Shih
Published: 202610.1007/978-1-0716-5104-9_6
Abstract
Our protocol outlines a DNA endonuclease-targeted CRISPR trans reporter (DETECTR) assay, which combines CRISPR/Cas12a technology with isothermal Recombinase Polymerase Amplification (RPA) for the rapid and specific detection of phytoplasma diseases i…
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