Methods in molecular biology (Clifton, N.J.)RNAFluorescence Resonance Energy TransferFluorescent DyesProtein BindingRNA-Binding Proteins
Analysis of Protein-RNA Interactions by Protein-Induced Fluorescence Enhancement (PIFE).
Joana Weiler, Véronique Arluison
Published: 202610.1007/978-1-0716-5084-4_6
Abstract
In this chapter, we provide an overview of a fluorescence technique, known as "Protein-Induced Fluorescence Enhancement" (PIFE), to follow the association between a protein and an RNA. Unlike Förster-type resonance energy transfer (FRET), which requi…
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