Methods in molecular biology (Clifton, N.J.)Cell Cycle ProteinsCohesinsChromosomal ProteinsNon-HistoneChromatin
ChIA-PET Analysis for Investigating SMC-Mediated 3D Genomic Contacts.
Kyoung-Dong Kim, Ken-Ichi Noma
Published: 202610.1007/978-1-0716-5005-9_16
Abstract
We present a detailed ChIA-PET benchwork protocol optimized for investigating cohesin- and condensin-mediated chromatin contacts. This method combines chromatin immunoprecipitation (ChIP), proximity ligation, EcoP15I digestion, and sequencing library…
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