Methods in molecular biology (Clifton, N.J.)AnimalsZebrafishCRISPR-Cas SystemsPhenotypeMutagenesis
CRISPR/Cas9-Based Mutagenesis Strategies for Efficient Biallelic Gene Inactivation and Consistent Phenotypic Detection in F0 Zebrafish.
Nathanael J Lee, Ryota L Matsuoka
Published: 202610.1007/978-1-0716-4985-5_18
Abstract
The zebrafish is a valuable animal model for investigating the genetic basis of vertebrate evolution, development, behavior, and regeneration. However, the existence of numerous gene paralogs in the zebrafish genome represents a major challenge, comp…
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