Methods in molecular biology (Clifton, N.J.)Two-Hybrid System TechniquesTranscription FactorsProtein Interaction MappingProtein BindingSaccharomyces cerevisiae
The Yeast Two-Hybrid System: In Vivo Detection of Direct Protein-Protein Interactions.
Pablo Morales-Martínez, Araceli G Castillo
Published: 202610.1007/978-1-0716-4972-5_8
Abstract
Yeast two-hybrid systems are one of the most popular and cost-effective in vivo genetic approaches for screening protein-protein interactions. The rationale behind the yeast two-hybrid (Y2H) system is the modular nature of transcription factors, whic…
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