Methods in molecular biology (Clifton, N.J.)Trypanosoma cruziGene EditingCRISPR-Cas SystemsDNA-Directed RNA PolymerasesViral Proteins
Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi.
Miguel A Chiurillo, Milad Ahmed, César González, Juliana Nunes Rosón, Asima Das, Noelia Lander
Published: 202610.1007/978-1-0716-4848-3_5
Abstract
The genetic manipulation of the human parasite Trypanosoma cruzi has been significantly improved since the implementation of the CRISPR/Cas9 technology for genome editing in this organism. Initially, the system was successfully used for gene knockout…
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