Methods in molecular biology (Clifton, N.J.)Microtubule-Associated ProteinsAutophagosomesHumansAutophagyBlotting
Monitoring Autophagosome Biogenesis and Degradation in Mammalian Cells Using LC3B Blotting.
Jennifer E Palmer, David C Rubinsztein
Published: 202610.1007/978-1-0716-4844-5_5
Abstract
Autophagy is a conserved lysosomal degradation pathway that recycles protein aggregates and damaged organelles to maintain cytoplasmic quality control. Measuring the amount of the lipid-conjugated autophagic protein LC3B-II is a useful way to test wh…
Preview only. Read the full abstract at the source