Methods in molecular biology (Clifton, N.J.)Hydrogen-Ion ConcentrationLysosomesFluorescent DyesHumansMicroscopy
Lysosomal pH Measurements via Dual-Imaging Microscopy.
Emily Kirkham, Sian Gardiner, Emyr Lloyd-Evans, Hannah Best, Helen Waller-Evans
Published: 202610.1007/978-1-0716-4844-5_2
Abstract
Dual-wavelength ratiometric fluorescent probes are the current gold standard for generating a quantitative measure of subcellular compartment ion contents. This chapter describes the use of fluorescent probes to measure lysosomal pH, encompassing pro…
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