Methods in molecular biology (Clifton, N.J.)Cryptosporidium parvumAnimalsMiceCRISPR-Cas SystemsCryptosporidiosis
Genetic Manipulation of Cryptosporidium parvum.
Sebastian Shaw, Adam Sateriale, Mattie C Pawlowic, Sumiti Vinayak, Carrie F Brooks, Jessica H Byerly, Boris Striepen
Published: 202610.1007/978-1-0716-4824-7_13
Abstract
Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9 driven homologous repair coupled to in vivo propagation in immunodeficient mice. This chapter will guide through foundational procedures for excystation, transfection, i…
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