Methods in molecular biology (Clifton, N.J.)CicerPolymerase Chain ReactionGenomicsPlant RootsDNA
TAIL-PCR for the Recovery of Tnt1 Flanking Sequences in Chickpea: A Tool for Functional Genomics Studies.
Chetan Chauhan, Shubhashish Ranjan, Vinod Kumar Jangid, Senjuti Sinharoy, Muthappa Senthil-Kumar, Divya Chandran
Published: 202610.1007/978-1-0716-4820-9_17
Abstract
Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) is a powerful technique for amplifying genomic regions flanking Tnt1-retortransposon insertions in plants. Here, we present a TAIL-PCR protocol for amplifying Tnt1-flanking genomic se…
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