Methods in molecular biology (Clifton, N.J.)Real-Time Polymerase Chain ReactionReference StandardsSaccharomycetalesGenesFungal
Establishing Reference Genes for Pichia pastoris Quantitative Real-Time Polymerase Chain Reaction Analyses.
Kai Büchner, Lana Vidal, Roland Kerpes, Thomas Becker
Published: 202610.1007/978-1-0716-4779-0_7
Abstract
Quantitative real-time polymerase chain reaction (qPCR) can be used to investigate the relative expression of selected genes under different conditions; hence, it requires a robust endogenous reference consisting of various constitutive genes, also k…
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