Methods in molecular biology (Clifton, N.J.)Protein Sorting SignalsRecombinant ProteinsSaccharomycetalesMixed Function OxygenasesPolysaccharides
Lytic Polysaccharide Monooxygenases as Reporters for Signal Peptide Evaluation in Pichia pastoris.
Andrea K Hönikl, Lukas Rieder
Published: 202610.1007/978-1-0716-4779-0_17
Abstract
Signal peptides are essential for the secretion of recombinant proteins using Komagataella phaffii. The N-terminal signal peptide is removed by proteases during the translocation process. However, this processing is not always complete, resulting in…
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