Methods in molecular biology (Clifton, N.J.)CloningMolecularSaccharomycetalesPolymerase Chain ReactionPlasmids
Overlap Extension Construction and Direct Cloning of Loop-Out Prone DNA Expression Cassettes in Pichia pastoris: 75 k-γ-Secalin from Secale cereale (rye) as a Case Study.
Kai Büchner, Carsten Pichler, Roland Kerpes, Thomas Becker
Published: 202610.1007/978-1-0716-4779-0_13
Abstract
Overlap extension PCR (OE-PCR) is a simple technique allowing for the construction of linear expression cassettes for direct integration into the Komagataella phaffii genome. This strategy enables the assembly and amplification of expression cassette…
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