Methods in molecular biology (Clifton, N.J.)
3D Spheroid Culture of Human Nasal Polyp Cells: A Functional Organoid-Like Model for Assessing Epithelial Restoration in Chronic Rhinosinusitis with Nasal Polyps (CRSwNP).
Nadia Lobello, Giovanna Lucia Piazzetta, Corrado Pelaia, Anna Di Vito, Silvia Di Agostino, Jessica Bria, Andrea Filardo, Mariaimmacolata Preianò, Isabella Coscarella, Nicola Lombardo, Emanuela Chiarella
Published: 202510.1007/7651_2025_683
Abstract
This chapter describes a reproducible protocol for establishing three-dimensional (3D) spheroid cultures from human nasal polyp epithelial cells obtained from patients with chronic rhinosinusitis with nasal polyps (CRSwNP). The model reproduces key f…
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