Small (Weinheim an der Bergstrasse, Germany)
Edge-State Programmed Carbonized Nanogels Enable Precise Hot-Start PCR by Multivalent Enzyme Inhibition.
Yu Tang, Binesh Unnikrishnan, Ju-Yi Mao, Chin-Jung Lin, Chen-Yu Lee, Chen-You Wang, Cheng-Yen Li, Han-Jia Lin, C Perry Chou, Chih-Ching Huang
Published: 202510.1002/smll.202512777
Abstract
Hot-start polymerase chain reaction (PCR) improves DNA amplification specificity by inhibiting polymerase activity before denaturation. We present carbonized polysaccharide nanogels (CNGs) as protein-free, thermoresponsive inhibitors for precise hot-…
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