Expression, Purification, and Microscopy-Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin.

Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications,…

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