Reconstruction of Efficient Flavin Adenine Dinucleotide (FAD) Regeneration Cycle to Enhance C1,2-Dehydrogenation of Steroids in Saccharomyces cerevisiae.

Flavin adenine dinucleotide (FAD) is an essential cofactor for numerous enzymes involved in critical physiological activities and industrial reactions. A notable example is 3-ketosteroid Δ1-dehydrogenase (KSTD), which catalyzes the C1,2-dehydrogenati…

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