Biotechnology and bioengineering
Engineered Stable, Antibiotic-Free, High-Level Protein Expression in the Probiotic Chassis Escherichia coli Nissle 1917.
Halimatun Sakdiah Zainuddin, Sanjeeva Kumar Murali, Thomas J Mansell
Published: 202510.1002/bit.70130
Abstract
The application of engineered live biotherapeutic products (LBPs) to secrete small molecules, peptides, or proteins to benefit a human or animal host, relies on heterologous protein expression. Key challenges in this area include expressing protein i…
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