Biotechnology journalHumansHEK293 CellsPlasmids12E7 AntigenFlow Cytometry
Intracellular Antibody Levels Predict Secretion Efficiency in HEK293T Cells Expressing a Chimeric Anti-CD99 Antibody from a Single-Expression Plasmid.
Manatchanok Chinakarapong, Kamonporn Kotemul, Sukrueta Hanprathet, Myint Myat Thu, Supansa Pata, Witida Laopajon, Watchara Kasinrerk, Nuchjira Takheaw
Published: 202510.1002/biot.70129
Abstract
Recombinant antibody production in mammalian cells is essential for therapeutic, diagnostic, and research applications. Single-expression plasmid systems that co-express both heavy and light chains under balanced promoters offer advantages in improvi…
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